Then click on the Literature (downloads only) to find the manual. On the Trans-Blot SD Semi-Dry Transfer Cell page click on the document tab below the picture of the system. ![]() Of protein may be transferred through the membrane and onto the filter paper below. Under the strong fieldsĭeveloped by this apparatus, transfers may not always be quantitative. The Trans-Blot® SD Semi-Dry Transfer Cell allows fast, efficient, economical blotting of proteins and nucleic acids without a buffer tank or gel cassettes. Recommended to prevent excessive heating during the run. The Trans-Blot SD semi-dry transfer cell allows fast, efficient, and economical blotting without a buffer tank or gel cassettes. A current limit (3 mA/cm2 for large gels 5.5 mA/cm2 for mini gels) is Large gelsĬan be transferred for 30 minutes to 1 hour at 15–25 V. Transfer mini gels for 15–30 minutes at 10–15 V. Concentrated, premixed anode and cathode buffers are available for purchase. This system uses 60 mM Tris, 40 mM CAPS, pH 9.6, plus 15 methanol in the filter paper on the anode side and 0.1 SDS on the cathode side. Although the Bio-Rad and Novex instructions don't seem to be that different (once again, see below) I think your best bet would be to follow the Novex instructions since they have probably optimized conditions for their apparatus.įrom the Trans-Blot SD Semi-Dry Transfer Cell, Rev C Instruction Manual (Part 1703940) A discontinuous buffer system using a Tris-CAPS buffer can greatly increase the efficiency of protein transfer by semi-dry blotting. If any defects occur in the instrument during this warranty period, Bio-Rad Laboratories will repair or replace the defective parts free. For most proteins, I usually transfer at 15 V for 25 - 30 min as recommended by Bio-Rad (see below). Bio-Rad Laboratories warrants the Trans-Blot SD semi-dry electrophoretic transfer cell against defects in materials and workmanship for 1 year. Includes base, 2 cassettes to hold 1-2 lunches or up to 4 mini blotter sandwiches, blotter roll.I occasionally use the Bio-Rad Trans-blot SD for my transfers. Traditional semi-dry transfer system transfers proteins ranging from 10100 kD in 1560 minutes. Environmentally friendly - environmentally friendly consumables eliminate disposal costs. Flexible design - allows user to customize transfer conditions and is compatible with traditional semi-dry consumables. Superior transfer efficiency - higher transfer efficiency than other protein transfer methods. ![]() Features and Benefits of the Trans-Blot Turbo Transfer System: - Fast blot transfer - 3 minute transfers of mini or midi gels - High throughput - can transfer 1-4 mini gels or 1-2 midi gels in one operation. ![]() Western blot of p-47 protein detected by. Consumables for protein blotting are available in ready-to-use, pre-packaged stacks or in bulk quantities requiring assembly. The Trans-Blot semi-dry transfer cell with the agarose gel support frame. Trans-Blot Turbo Transfer Packs include an optimized combination of buffer, membrane, and filter paper that provides superior transfers when paired with the Trans-Blot Turbo blotting device. The system enables protein transfer in just 3 minutes without sacrificing performance compared to traditional in-tank protein transfer. The Trans-Blot Turbo Transfer System is a high performance western blotting transfer system designed to provide rapid transfers with high efficiency.
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